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Lipid analysis: Isolation, separation, identification and lipidomic analysis (Fourth edition)W W Christie, Scottish Crop Research Institute and Mylnefield Research Services, UK and X Han, Washington University School of Medicine, USA
Oily Press Lipid Library Series No. 24
WW Christie was the winner of the `2010 Supelco/Nicholas Pelick - AOCS Research Award'
This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and ‘lipidomics’ as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf.
Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics.
The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.
ISBN 0 9552512 4 9
ISBN-13: 978 0 9552512 4 5
January 2010
448 pages 234 x 156mm hardback
£145.00 / US$245.00 / €175.00
Usually dispatched within 24 hours
About the authors
William W. Christie, Scottish Crop Research Institute and Mylnefield Research Services, Scotland.
Xianlin Han, Washington University School of Medicine, USA.
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Contents
PART 1 INTRODUCING LIPIDS AND THEIR ANALYSIS
PART 2 CHROMATOGRAPHIC SEPERATION AND ANALYSIS OF INDIVIDUAL LIPID CLASSES
PART 3 ANALYSIS OF FATTY ACIDS
PART 4 CHROMATOGRAPHIC ANALYSIS OF MOLECULAR SPECIES OF LIPIDS
PART 5 MASS SPECTROMETRIC ANALYSIS OF LIPIDS IN LIPIDOMICS
PART 1 INTRODUCING LIPIDS AND THEIR ANALYSIS
Lipids: their structures and occurrence
- Introduction
- Definitions
- Fatty acids
- Principle simple lipids and complex glycerolipids of animal and plant tissues
- Sphingolipids
Chromatographic analysis of lipids: general principles
- A statement of the problem
- The theory of chromatoghaphy
- Gas chromatoghaphy
- High-performance liquid chromatoghaphy
- Thin-layer chromatoghaphy
- Supercritical fluid chromatoghaphy
- Mass spectrometry
- Quality control
Lipid extraction, storage and sample handling
- Introduction
- Some practical considerations
- The principles of solvent extraction procedues
- Recommended procedues
PART 2 CHROMATOGRAPHIC SEPERATION AND ANALYSIS OF INDIVIDUAL LIPID CLASSES
Analysis of simple lipid classes
- Preliminary fractionation of lipid extracts
- Low-pressure column chromatoghaphy
- Thin-layer chromatoghaphy
- High-performance liquid chromatoghaphy
- Delamination of lipid profiles by gas chromatoghaphy
- Analysis of specific simple liquid classes
- Oxidized fats and oils
Chromatographic analysis of phospholipids and glycosyldiacylglycerols
- Introduction
- Separation by low-pressue column chromatoghaphy
- Thin-layer chromatoghaphy
- High-performance liquid chromatoghaphy
- Quantification of complex lipids separated by chromatoghaphy
- Phosphorus nuclear magnetic resonance spectroscopy
- Some specific problems in the analysis of complex glycerolipds
Chromatographic analysis of sphingolipids
- Introduction
- Preparation of Sphingolipid-rich extracts
- separation of neutral glycosphingolipid classes
- Separation of gangliosides
- analysis of hydrolysis products of glycosphingolipids
- Come miscellaneous glycolipids
PART 3 ANALYSIS OF FATTY ACIDS
Preparation of derivatives of fatty acids
- Introduction
- Hydrolysis 9saponification) of lipids
- Preparation of methyl and other esters of fatty acids
- Preparation of derivatives for mass spectrometry
- Derivatives of cdouble bonds
- Derivatives of hydroxyl groups
Gas chromatographic analysis of fatty acid derivatives
- Introduction
- Columns and lipid phases
- Provisional identification using standards or retention time relationships
- Separation of the common fatty acids of plants and animals
- Positional and geometric isomers of unsaturated fatty acids
- Quantitative estimation of fatty acid compositions
Isolation of fatty acids and identification by spectroscopic and related techniques
- Introduction
- Simple concentration techniques
- Silver ion chromatoghaphy
- Reversed-phase high-performance liquid chromatoghaphy
- Adsorption chromatoghaphy
- Identification of fatty acids by chemical degradative procedures
- Spectroscopy of fatty acids
- Mass spectrometry
PART 4 CHROMATOGRAPHIC ANALYSIS OF MOLECULAR SPECIES OF LIPIDS
Molecular species of triacylglycerols, diacylglycerols derived from complex lipids, and related lipids
- Introduction
- Separation of triacylglycerol species
- Molecular species of mono- and diacylglycerols
- Other simple lipids
Chromatographic analysis of molecular species of intact phospholipids and glycolipids
- Introduction
- Hig-performance liquid chromatoghaphy separation of intact phospholipids
- Glycoglycerolipids
- Glycosphingolipids
Positional distributions of fatty acids in glycerolipids
- Introduction
- Positional distribution of fatty acids in triacyl-sn-glycerols
- Enzymatic hydrolysis of complex lipids
PART 5 MASS SPECTROMETRIC ANALYSIS OF LIPIDS IN LIPIDOMICS
Introduction to mass spectrometric analysis of lipids in lipidomics
- Lipidomics
- Brief history of mass spectrometry and lipid analysis
- Modern developments in mass spectrometry for lipid analysis
- Tandem mass spectrometry modes useful for lipid analysis in lipidomics
- Impact of recent developments related to mass spectrometry analysis of lipids in lipidomics
Characterization of lipids by electrospray ionization mass spectrometry
- Introduction
- Characterization of individual classes of Phospholipids
- Characterization of individual classes of sphingolipids
- Characterization of simple glycerolipids and glycosyldiacylglycerols
Practical identification of individual lipid species in lipid extracts of biological samples
- Introduction
- LC-MS based identification of individual molecular species of lipid classes
- Identification of individual molecular species of lipid classes shotgun lipidomics
- Location of double bonds in individual lipid species
Quantification of lipid molecular species by electrospray ionization mass spectrometry
- Introduction
- Internal standards and normalization
- Quantification of lipids by HPLC-coupled ESI-MS and ESI-MS/MS
- Quantification of of lipids after direct infusion
- Concerns associated with quantification
- Conclusion